This training program will provide
trainees with needed basic knowledge as well as extensive practical skills for
bright field microscope (compound microscope) handling; and subsequently raise
their abilities in microscope calibration & proper use of eyepiece micrometer
to identify parasites especially ova & cysts.
Intended
learning outcomes (ILOs):
1. Understand the
principles & precautions needed for proper handling of compound microscope.
2. Perform a range
of appropriate training in the lab to use different magnifications especially
oil immersion lens for parasites identification.
3. Work out with
extensive training of: how to do microscope calibration?
4. Use of ocular
micrometer for measuring different parasitic stages that may be recovered from
stool, urine and soil.
5. Describe
scientifically the important morphological features of recovered parasites
Importance of the program:
Correctly calibrated microscope and
accurate measurement is crucial for parasitologist because size is an important
characteristic for identification of different parasites. This program is essential in providing needed
training in microscopy & practice of ocular micrometer for precise
measurement of parasitic stages.
Program
regulation and outline:
Each student is introduced with a
short theoretical session on bright field microscope parts, proper care &
performance, high lightening how to obtain best images with a special
consideration for oil immersion lens. Practical sessions are performed on
microscope calibration & using the micrometer for precise measurement. Practical work is done through group
work. Practical sessions are concluded
by discussions, demonstration and grading of the best performing group of the
day. The trainees will be asked to incorporate in activity by applying the
procedure to measure different parasitic stages (e.g. eggs of helminths,
trophozoites & cysts of protozoa).
Main Topics:
- Introduction to
compound Microscope.
- Microscope
parts, oil immersion lens.
- How to get best image?
- What is micrometry?
- Why it is important
to use it?
- Scientific
description of microscopic images.
- How to do microscopic calibration (4X & 10X magnification)?
And to use micrometry in precise
measurement?
- How to do microscopic calibration (40X & 100X magnification)?
And to use micrometry in precise
measurement?
- Practical application on diversity of slides (helminthes, protozoa
& arthropods).
- Practical
Evaluation
The teaching strategy
is based on extensive laboratory work. All students must be well trained
on the method of calibration of different magnification powers using ocular
micrometer. Then they must be as well skilled on using the ocular micrometer
for measuring the size of recovered parasitic stages.
During the practical sessions trainees will do & practice all skills that
have been taught. Trainees are divided
into groups of two or more according to their number. At the end of the session, test results are
compared and problems are discussed.
Learning Time:
This program depends mainly on training
sessions with few theoretical lectures and the learning time is designed as
follows;
o
Lecture represents only 8.3% of the learning
time (7.5 hours) and 36.7% (33 hours) is for tutorial & practical sessions
(contact time is 45%).
o
45 % (40.5 hours) is for self-directed
training.
o
The remaining 10% (9 hours) is
specified for assessment.
Depends mainly on practical
achievement