Aim: To provide a basic understanding and practical experience of a range of reagents, methods and tools of the molecular techniques. The program also aims to demonstrate what can be achieved with the molecular approach, and how expertise in this area can be integrated into career development. Practical training will be built around an appropriate research theme. Intended learning outcomes (ILOs): By the end of this module students should be able to: 1. Demonstrate knowledge and understanding of the principles underpinning important techniques in molecular biology. 2. Demonstrate knowledge and understanding of some applications of these techniques in laboratory research on parasitic diseases. 3. Demonstrate the ability to carry out basic laboratory experiments in molecular biology with analysis and interpretation of the results. 4. Demonstrate the ability to read, comprehend and assess the relevant scientific literature. Importance of the program:
This program is intended for our students who require theoretical knowledge and practical experience of standard molecular biology techniques which is crucial for advanced recent researches.
Program regulation: This program is designed for students with a basic knowledge of molecular biology. Concurrent lecture topics will be closely linked to the practical work. Following the practical section of the module, published papers will be read and discussed to illustrate applications of the techniques covered. Revision sessions are included to consolidate the theoretical aspects of the program. Main topics: - DNA structure and function - Different DNA extraction techniques - Different methods for visualization of nuclear products - Principles of DNA amplification - Conventional PCR techniques - Quantitative real time PCR technique - Principles and application of genotyping in Parasitology - Principles and application of gene expression in Parasitology - Principle of cloning technique and its application in Parasitology field Teaching Strategy and Learning time: The program is a mainly practical, intended to develop laboratory skills and experiences through the following teaching strategies: o Theoretical lectures o Practical sessions with interactive discussions o Critical analysis and appraisal of scientific researches related to molecular technology Learning Time:
o Lecture represents 20% of the learning time and 40% is for practical sessions (contact time is 60%). o 20 % is for directed self-study and self-directed learning. o The remaining 20% is specified for assessment, review and revision. Assessment: Assessment will be based on a written report, in scientific paper format, on one of the practical sessions. Some consideration will be given to the quality of data provided. |
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